“Gone! It’s all gone! All of its gone, bye-bye, woo, see ya! What happened to you? One minute you’re defending the whole galaxy! Then suddenly you find yourself sippin’ down Darjeeling with Marie Antoinette and her little sister (giggle). I think you’ve had enough tea for today, Buzz. Let’s get you outta here. Don’t you get it? You see the hat? I AM MRS. NESBIT! AHHAHAHAHA! Snap out of it, Buzz! (click, squeak, click) I’m sorry. You’re right. I’m just a little depressed, that’s all. I can get through this. Oh, I’m a sham!! Shh, Buzz. Can’t even fly out of a window! But the hat looked good, tell me the hat looked good. The apron was a bit much, it’s not my color… Out the window! Buzz, you’re a genius! Come on this way. Years of academy, training wasted!” and “Look, I’m Woody! Howdy, howdy, howdy! AHHAAHHA, give me that.”
I just LOVE that one! Laugh everytime I watch it!! 
After Zurg falls to his “death”:
Buzz #2: …father.
I also liike (in Andy’s mums car)
‘I’m Saved!, I’m Saved!’
‘Andy will find you here, he’ll take us back to the room, and then you can tell everyone this was all just a big mistake’
‘Huh?, Riiight? … Buddyyy 
?’
This is one of my favourite little moments from the original Toy Story film, the quote I like is in bold.
Hamm: So,er, where you from? Singapore? Hong Kong…
Buzz: Well, as a matter of fact I’m stationed up at the Gamma quadrant of Sector 4, as a member of the elite Universe Protection Unit of the space ranger corps, I protect the galaxy from the threat of invasion from the Evil Emperor Zurg, sworn enemy of the Galactic Alliance!
Mr. Potato Head: Oh really? I’m from playschool!
Rex: and I’m from Mattel! Well actually I’m from a smaller bla bla bla…
“I Can’t believe I have to drive all the way to work on a Saturday; all the way to work!”
Toy Story has so many great lines…but so far, I think this is the funniest between the two films:
“Buzz! Buzz, are you okay?”
“GONE! It’s all gone. All of it’s gone! Bye-bye, Hoo-hoo, See ya!”
“What happened to you?”
“One minute you’re defending the WHOLE GALAXY, and then SUDDENLY, you find yourself SUCKIN’ DONE DARJEELING with…Marie Antoinette and her little sister…chuckles”
“I think you’ve had enough tea for today…let’s get you out of here, Buzz–”
“Don’t you GET IT? DO YA SEE THE HAT? I AM MRS. NESBITT!!” insert mad cackling here
“SNAP OUT OF IT, BUZZ!”
“I, I, you’re right, I’m sorry…I am just a little depressed, that’s all…I can get through this…OH, I’M A SHAM!!!”
Buzz going mad is always hilarious.
From the first film:
“The word I’m looking for, I can’t say, because there are preschool toys present.” - Woody
The infamous gas station scene.
“I don’t believe that man has ever been to medical school.” - Buzz at Sid’s house
“You see the hat?! I am Mrs. Nesbit!” hysterical laughter - Buzz with Hannah’s toys
And the second:
“NONONONOOOOOOOOOOO!” - Al, when Woody loses his arm
“If. The. Boot. Fits.” - Woody, just before he fights with Jessie
“Welcome to Al’s Toy Barn.” sniffles “We’ve got the lowest prices in town. Everything for a buck-buck-buck.” sobs - Al
These are just the one’s from the top of my head right now.
Mr Potato Head- “You would not Believe the day I’ve had”
Barbie- “You are so smart!”
Ken- “See, it changes every time!”
Lotso- “I’m a hugger!”, “You got a playdate with destiny!”
Buzz- “Buzz Lightyear Al Rescate!”
-Omar
I see by reading through this one of my fav quotes has been mentioned at least twice. That one being the one that’s currently in my sig 
I’m not sure if I can come up with a different one, as so many of my favs have been mentioned. Let’s see, sorry if these have been said already:
Buzz- Unidirectional bonding strip
Robot- Mr Lightyear wants more tape!
Buzz- How dare you open a spaceman’s helmet on an uncharted planet! My eyeballs could have been sucked from their sockets!
Sid- Extremely dangerous, keep out of reach of children. Cool!
Some of mine from Toy Story 3
[spoiler]Jessie-We’re not a family. It is a prison. You’re a liar and a bully. I rather rot in this dumpster, then be in any family of yours.
Barbie-Jessie’s right. Authority should derive from the consent of governed. not from the threat of force
Ken-Barbie, wait. Don’t do this, Lotso.
Lotso-She’s a Barbie doll, Ken. There’s a hundred million just like her.
Ken-To me. there’s not.
Ken-Everyone, listen. Sunnyside could be cool and groovy if we treat each other fair. it’s Lotso. He made us all into a pyrmaid and put himself on the top
Big Baby-Mama[/spoiler]
Buzz: "I cant even fly out of window! The hat looks good, tell me the hat looks good. The aprons a bit much.
Woody: “Out the window, Buzz your a genius!”
Buzz: “YEARS OF ACADEMY TRAINING WASTED!”
Bo: “Andy will always have a special place for you.”
Potato Head: “Yeah like the attic!”
From the commentary of TS2, didn’t know who said it. He was talking about Zurg
“I’m so proud our villain wears a skirt.”
monte73 you beat me to it. Years of academy training wasted!
My fave quotes are:
Buzz:
I am Mrs. Nesbit!
Woody:
I am officialy freaked out…
Potato Head:
What are you looking at, you hockey puck?
You could not believe what I have been through!
Hey Hamm, I’m Picasso!
Mrs. Potato Head:
DON’T TALK TO ANY TOYS YOU DON’T KNOW!
Mr. Spell:
[Mrs. Potato Head is called out as a gift and Mr. Spell says Hubba Hubba]
-“The word I’m looking for I can’t say, because there are preschool toys present.”
- The whole Mrs. Nesbitt speech
- “Who’s behind?” “Mine.”
- “I’m a married spud, I’m a married spud, I’m a married spud…”
- “I seriously doubt he’s getting this kind of milage.”
- “I was a yo-yo!” “‘Was’?”
- “Buzz, can you give me a hand?” Buzz throws arm “Ahahaha, very funny. THIS IS SERIOUS!”
- “What are you looking at, ya hockey puck?”
There are lots of great quotes in these movies (I haven’t seen the 3rd one yet)
Buzz : “HAHHHHKHH MY SHIEEHPP!”
thats gorgeous haha
Buzz : “my eyes gonna sssucked from their ssssocket!”
[spoil]After Buzz was re-reset in TS3, he sniffs and says, “That wasn’t me, was it?” 
[/spoil]
TS1:
Rex: What if Andy gets another dinosaur? A mean one? I just don’t think I can take that kind of rejection!
Buzz: I’ve set my laser from stun to kill.
Woody: Oh, great. If anyone attacks we can blink em’ to death.
Woody: The word I’m searching for, I can’t say, because there’s preschool toys present.
Mr. Potato Head: Did you all take stupid pills this morning?
TS2:
Woody: Your right, Prospector. I can’t stop Andy from growing up… but I wouldn’t miss it for the world.
Buzz # 2: Quick, help me prop up Vegetable man here or we’re done for!
Barbie Doll: Hi, you’ll like Amy. She’s an artist!
Rex: Don’t do it, Woody! We love you!
Woody: Lucky? Are you shrink-wrapped? I am missing my ARM!
Rex: Guys, we can’t park here. It’s a white zone.
TS3
[spoil]Buzz Lightyear: Spare me your lies, temptress! Your emperor’s defeated, and I’m immune to your bewitching good looks.
Buttercup: There is no way out!
Woody gasp
Buttercup: Just kidding. Door’s right over there.
Woody: "I’d like to join your posse boys, but first I’m going to sing a little song
Ken: “And this is where the magic happens”
Buzz Lightyear: Quiet musical hog!
Mr. Potato Head: Jessie’s right, Woody. She was wrong. [/spoil]
From the first movie,
“Terrain seems a bit…unstable”
(i like how he said it )
[spoil]Mr Potatohead: “Hey! No one takes my wife’s mouth! Except for me… now give it back, you furry air freshener!”[/spoil]
That one made me happy.